Photoinactivation of Aldolases by Pyridoxal Phosphate and Its Analogues
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چکیده
منابع مشابه
Photoinactivation of aldolases by pyridoxal phosphate and its analogues.
Pyridoxal phosphate can act as a specific photosensitizer for amino acid residues in rabbit muscle and spinach leaf aldolases, but the residues affected depend on the pH of the reaction. Below pH 8 one histidine residue per enzyme subunit is destroyed; above pH 8.5 there is little loss of histidine, and photoinactivation is associated with the destruction of specific tyrosine residues, particul...
متن کاملPyridoxal phosphate-sensitized photoinactivation of glutamate decarboxylase from Clostridium perfringens.
1. l-Glutamate decarboxylase (EC 4.1.1.15) from Clostridium perfringens was inactivated by exposure to visible light at pH6.2. 2. Inactivation does not occur at pH4.6 or in the absence of bound pyridoxal phosphate. 3. On prolonged photo-oxidation six histidine residues per molecule of enzyme were destroyed. 4. The loss of six cysteine residues per molecule occurred both in irradiated samples an...
متن کاملMOLECULAR MODELING AND NMR STUDY OF HISTDINIE AND ITS ANALOGUES AS , PYRIDOXAL 5 '-PHOSPHATE DEPENDENT HISTIDINE DECARBOXYLASE INHIBITORS
Molecular modeling analysis of charge density and heat of fornation by PM3 method as well as C, H NMR and 2D-NMR measurements of histidine (substrate) and some of its derivatives as histidine decarboxylase inhibitors were performed. It was established that the atom, usually nitrogen, which forms internal aldimine with pyridoxal5 -phosphate (PLP), (coenzyme), has negative and almost equal ...
متن کاملmolecular modeling and nmr study of histdinie and its analogues as , pyridoxal 5 '-phosphate dependent histidine decarboxylase inhibitors
molecular modeling analysis of charge density and heat of fornation by pm3 method as well as c, h nmr and 2d-nmr measurements of histidine (substrate) and some of its derivatives as histidine decarboxylase inhibitors were performed. it was established that the atom, usually nitrogen, which forms internal aldimine with pyridoxal5 -phosphate (plp), (coenzyme), has negative and almost equal charge...
متن کاملBinding of Pyridoxal 5'-Phosphate
1. The a and ,B subforms of aspartate aminotransferase were purified from pig heart. 2. The a subform contained 2mol of pyridoxal 5'-phosphate. The apo-(a subform) could be fully reactived by combination with 2mol of cofactor. 3. The protein fluorescence of the apo(a subform) decreased non-linearly with increase in enzyme activity and concentration of bound cofactor. 4. It is concluded that the...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1971
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.68.2.416